Extended Data Fig. 8: Characterization of mice with HSC-, EC-, hepatocyte and KC-specific Rspo3 deletion.
From: Hepatic stellate cells control liver zonation, size and functions via R-spondin 3
a. qPCR for the indicated genes in livers from Rspo3 fl/fl (n = 8) and Rspo3 ΔHSC (n = 8, 7 male, 1 female) mice. b. IHC, morphometric quantification and qPCR for the indicated zonation markers in Rspo3 fl/fl and Rspo3 ΔHSC mice (n = 8/group). c. Zonal quantification based on IHC from b (n = 8). d. Wnt-focused 100plex spatial transcriptomics and cluster-based zonal quantification in Rspo3 fl/fl (n = 1) and Rspo3 ΔHSC (n = 1) mice. e-f. qPCR for Rspo3, Wnt2 and Wnt9b in ECs from Rspo3 ΔHSC (n = 4) (e) and Rspo3 ΔHSC-ind (n = 5) (f) mice or Rspo3 fl/fl littermates (n = 4). g-i. Hepatic Rspo3 mRNA and liver-body weight ratio in Rspo3 ΔHSC-ind (n = 5) (g), Rspo3ΔHep (n = 4) (h) and Rspo3ΔKC (Rspo3 fl/fl n = 5, Rspo3 ΔKC n = 4) (i) mice or floxed controls. Data are shown as mean ± s.e.m. Each dot represents one biological replicate (a-b,e-i). Scale bars 100 µm (c,k,l,m,n,o), 100 µm (d, white), 1 mm (d, black). P-values were calculated using unpaired two-tailed t-tests (a-c,e-i).
