Extended Data Fig. 9: Liver zonation in mice with HSC-, EC-, hepatocyte and KC-specific Rspo3 deletion.
From: Hepatic stellate cells control liver zonation, size and functions via R-spondin 3
a-d. IHC, morphometric quantification and qPCR of zonation markers and, in some cases, zonal quantification of IHC in livers from Rspo3 ΔHSC-ind (n = 7) or Rspo3 fl/fl (n = 6) (a), Rspo3 ΔHep (n = 4) or Rspo3 fl/fl (n = 4) (b), Rspo3 ΔKC (n = 4) or Rspo3 fl/fl (n = 5) (c), and Rspo3 ΔEC (n = 8) or Rspo3 fl/fl (n = 6) (d) littermates. e. Rspo3 qPCR in ECs (n = 5), the liver-body weight ratio (Rspo3 fl/fl n = 6, Rspo3 ΔEC-ind n = 7), IHC, morphometric quantification of zonation markers CYP2E1 and CYP1A2 (Rspo3 fl/fl n = 6, Rspo3 ΔEC-ind n = 7), CYP2F2 (Rspo3 fl/fl n = 13, Rspo3 ΔEC-ind n = 12) and HAL (Rspo3 fl/fl n = 13, Rspo3 ΔEC-ind n = 12) in livers of Rspo3 ΔEC-ind mice. Data are shown as mean ± s.e.m. Each dot represents one biological replicate (a-e). Scale bars 100 µm (a-e), P-values were calculated using unpaired two-tailed t-tests (a-e).
