Extended Data Fig. 7: LAR effectively kills A. baumannii C0286 in vitro and ex vivo.
From: A broad-spectrum lasso peptide antibiotic targeting the bacterial ribosome
(a) In vitro time-kill assay in MHB medium showing the bactericidal effect of LAR as denoted by reduction in viable cfu counts. Data are plotted as the mean of three biological replicates with the error bars indicating SD. (b) Cidality of LAR in the ex vivo model. A. baumannii C0286 was inoculated in human blood, and bacterial cfu were enumerated after 4 h treatment with LAR. Data are plotted as mean ± SD of three biological experiments. Significance was determined using one-way ordinary ANOVA with Dunnett’s multiple comparisons test. (*P = 0.0118; **P = 0.0017). (c) MIC of LAR against A. baumannii C0286 in MHB with or without the addition of serum. FBS=fetal bovine serum; HS=human serum (heat inactivated).
