Extended Data Fig. 6: Elevated TNF affects the expression of fibrinolysis-related genes in endothelial cells.
From: Clinically relevant clot resolution via a thromboinflammation-on-a-chip
RT-qPCR results show exposure to 10 ng ml−1 TNF significantly reduces the expression of PLAT (encoding tPA), PLAU (encoding uPA), and/or PLAUR (encoding uPAR), while the expression of SERPINE1 (encoding PAI-1) and SERPINB2 (encoding PAI-2) is either less affected or increased in four types of primary human endothelial cells cultured under static condition. (n = 4 biological replicates for HUVECs. n = 3 biological replicates for cardiac, dermal, and glomerular microvascular endothelial cells. Data are shown as fold change compared to gene expression in control endothelial cells without TNF stimulation and are presented as mean values +/− SD. p values were calculated using a two-sided t-test between TNF-treated and control endothelial cells).
