Extended Data Fig. 8: Recombinant human neutrophil elastase induces endothelial detachment and apoptosis in a dose-dependent manner.
From: Clinically relevant clot resolution via a thromboinflammation-on-a-chip
a, Representative epi-fluorescence images with or without overlaying with phase-contrast images demonstrate that 690 nM recombinant human neutrophil elastase results in HUVEC detachment and apoptosis after a 3-hour culture. b, High magnification images of the boxes in (a). c, Representative epifluorescence images with or without overlaying with and phase-contrast images demonstrate that a 1-day culture with 690 nM human neutrophil elastase results in complete HUVEC detachment and cell fragmentation, while a 1-day culture with 345 nM elastase results in HUVEC detachment and apoptosis, similar as that observed after a 3-hour culture with 690 nM elastase. d-e, Higher magnification images of the boxes in (c). [(scale bar = 100 μm (a-d)].
