Extended Data Fig. 6: Pseudo-spatial profiling of mZ-HLOs show similarity of zonal expression to primary liver tissue.
From: Multi-zonal liver organoids from human pluripotent stem cells
a) Spatial plot for TAT (zone 1), HAMP (zone 2), and CYP3A4 (zone 3) markers in 10X Xenium healthy human liver dataset (publicly available dataset). b) UMAP plot of mZ-HLO with hepatocyte populations (top) and distribution of replicate data (bottom). c) Feature plot for TAT (zone 1), HAMP (zone 2), and CYP3A4 (zone 3) markers. d) UMAP plot for zonal hepatocyte populations from primary liver (Andrews et al.30) and mZ-HLOs integrated together (top). UMAP plot depicting distribution for total hepatocyte populations from primary liver and mZ-HLOs integrated together (bottom). e) Expression of known hepatoblast and zonal hepatocyte marker genes in mZ-HLOs benchmarked against Andrews et al.30 snRNAseq dataset. f) UMAP plot for all cell types (inset: sample distribution) from primary liver datasets and mZ-HLOs integrated together. g) Feature plot for GLS2 (zone 1), HAMP (zone 2), and GLUL (zone 3) markers.
