Extended Data Fig. 10: Similar response profiles in mice with different depilation timing.

We used three different populations of mice during this study because we needed the fur intact to assess brushing and depilated skin for temperature series. Data in Figs. 1 and 2 were obtained from mice where the fur was removed acutely (during the functional imaging after mechanical stimulation had been performed). Data in other figures either used no fur removal (brush studies) or depilation was the previous day (approx. 24 h before recording) to minimize potential irritation from the chemical fur removal needed for temperature series. Note studies of inflammation were all carried out without acute fur removal. Response magnitudes (AUC mean ± s.e.m.) for same day (pale grey) and previous day (dark grey) depilation regimes for the different cell classes indicate only minimal differences in sensitivity; p < 0.05, *; p < 0.01, **; p < 0.001, ***. For details of statistical tests and numbers of mice and cells see Supplementary Information, Tables 1 and 2.