Extended Data Fig. 4: Tuning of NP1 neurons reflects stimulus application rather than heterogeneity in this class of sensory neurons.

Heatmaps showing Ca-transients from trigeminal neurons of three Ai95 mice carrying the Mrgprd-CreER knockin allele after tamoxifen induction; changes in GCaMP fluorescence in response to stimuli applied to the cheek are color-coded by % ΔF/F. Responding neurons were sorted based on their response to heating. Note that for each mouse, there were individual pinches that primarily activated neurons that were not temperature responsive (an example boxed, green) and other pinches that generally activated temperature responsive neurons (an example boxed, red). This strongly suggests that all NP1 neurons innervating the cheek are polymodal and that differences in position of stimulus activation or size of thermal versus mechanical receptive field account for apparent variation in tuning selectivity.