Extended Data Fig. 5: Analysis of the chronically stressed brain by Stereo-seq.
From: Psychedelic control of neuroimmune interactions governing fear
a, H&E and nuclei staining of coronal brain sections used in Stereo-seq. b, RIN number of samples used in Stereo-seq analyses. n = 3 per group. Unpaired t-test. c, Rarefaction curve of sequencing depth for each sample used. d, Abundance of RNA molecules detected per spot at a resolution of bin200, meaning a 100 µm2 spot. Please note that Stereo-seq transcriptomic data were analyzed using cellbin analysis, which maintains a resolution of 500-nm. e, Representative tissue architecture for sections used. f, Representative mID counts in an 18-day stressed mouse at cellbin resolution (500-nm). RIN, RNA integrity number. Data shown as mean ± SEM.
