Extended Data Fig. 10: Control analyses of human samples.
From: Psychedelic control of neuroimmune interactions governing fear
a, Normalized expression of genes detected by ultra-low-input bulk RNA-seq from human immune cell populations in the blood from the ImmGen human immune system cell atlas. b, Pathways detected by GSEA in primary human monocytes treated for 6-h with 100 ng/mL LPS with or without 10 µM psilocybin or 10 µM MDMA racemate. n = 3 per group. c,d, Demographics of patient samples used for snRNA-seq. n = 6 per group. Unpaired t-test (c) or Fisher’s exact test (d). e, Violin plots of cell type markers for cells detected in the amygdala by snRNA-seq. f, Separation by disease status of samples used in snRNA-seq. g, Pathway plots of cluster 1 and 6 astrocytes from snRNA-seq. h, qPCR analysis of EGFR expression in human amygdala. n = 6 per group. One-tailed unpaired t-test. i, Representative confocal images and bar graphs showing EGFR expression in control and MDD patient amygdala astrocytes. n = 7-10 images and n = 3 patients per group. Unpaired t-test. j, Violin plot of NR2F2 and SLITRK2 expression in excitatory neurons. In violin plots, Solid horizontal line: median; white box: interquartile range (25th–75th percentile); whiskers: min and max values; dashed line: mean. Data shown as mean ± SEM.
