Extended Data Fig. 3: RNAi screen in Drosophila.
From: Structural basis of lipid transfer by a bridge-like lipid-transfer protein
a, Mass spectrometry analysis of the LPD-3 complex, showing the proteins that were subjected to RNAi-mediated knockdown in Drosophila astrocytes to in order to identify regulators of phagocytosis. Y38C1AA.12 lacks a gene name in C. elegans and is referred to in this text as LTAP2. pSC = peptide spectral counts. For the sequences of each RNAi construct, see Supplementary Table 2. b, Overview of vCrz neuron morphology: Eight pairs of vCrz neurons are present in the ventral nerve cord, red dotted box represents imaged region. c, vCrz neuronsat the wandering third instar larval stage (wL3) in controls which is broken down and fully cleared by astrocytes at head eversion (HE, ~12 h after puparium formation) in controls. Astrocyte knockdown of spigot (CG6665) with GMR25H07-Gal4 (astrocyte-specific driver) does not affect neuronal morphology at wL3 but disrupts the ability of astrocytes to clear neuronal debris by HE. vCrz neurons were labelled with anti-Crz, and all images are maximum Z projections of the entire ventral nerve cord (VNC). Scale bar, 50 μm. d, Quantification of data from (c) for controls (UAS-FLP5.DD) (wL3, N = 7; HE, N = 12) and UAS-spigot RNAi (wL3, N = 12; HE, N = 5). Graphs show mean ± SEM, and each dot represents independent animals. Statistical comparisons were performed using two-way ANOVA with Sidak’s multiple comparisons test. ****P < 0.0001, ns=not significant.
