Extended Data Fig. 8: Primary tumor construction by p53 and Brca1 knockout using NanoFLUID-mediated transfection.
From: A battery-free nanofluidic intracellular delivery patch for internal organs
a, WB of protein p53 and BRCA1 in the E0771 cells transfected with p53 and Brac1 knockout (KO) plasmids. Control: NanoFLUID-mediated transfection of piggyBac transposon plasmid containing non-target gRNA sequences. b, Photograph of a moving mouse after NanoFLUID implantation (zoomed-in area) and during wireless transfection treatment. c, Representative sequencing chromatograms showing successful knockout of Brca1 and p53 in the genome. Red arrowheads and boxes indicate knockout sites and the corresponding nucleotides deleted from the wild-type reference genome, respectively. Sequence comparison between the knockout and reference genomes is shown below. d, Flowchart of NanoFLUID in vivo transfection, whole-tissue clearing and 3D imaging and reconstruction of the mammary gland. e, 3D image of a mammary gland showing the distribution of transfected, GFP-expressing cells one month after NanoFLUID transfection. f, Tumorigenesis and metastasis rate of mice 60 weeks after in vivo transfection by NanoFLUID or liposome. n = 15 mice per group. g, H&E staining of the tumorigenic mammary glands and lungs with metastatic sites 60 weeks after transfection by NanoFLUID or liposome.
