Extended Data Fig. 10: Gene library construction from TCGA.
From: A battery-free nanofluidic intracellular delivery patch for internal organs
a, Volcano plot of differentially expressed gene between tumor samples from patients with or without metastasis. Expression levels with statistically significant increase or decrease (p < 0.05) are labeled in red and blue, respectively. b, 362 genes associated with the worst prognostic outcomes (hazardous ratio (HR) > 1) were filtered out from the 503 significantly upregulated genes in (a). c, Forest plot of the top 50 genes among the 362 genes ranked by HR based on Cox regression analysis. d, Gene Set Variation Analysis (GSVA) of the 50-gene collection between tumor samples from patients with or without metastasis. e, Box plot of the expression level of the 50 genes in tumor samples obtained from TCGA patients. All 50 genes are upregulated in tumor samples compared to healthy tissue (normal) and are overexpressed in tumor samples from patients with metastasis compared to those without. f, Heat map of the abundance of the 50 genes in 500 GFP-positive cells (each row) isolated from the lungs and livers of PyVmTLibrary mice. g, IGV plot of the enriched genes in (f). h, IGV plot of the enriched genes in (Fig. 6i). i. Cellular component enrichment analyses of the proteins upregulated in cancer cells from PyVmTDUS2 mice relative to PyVmTEmpty mice. Results are mean ± s.d. (c) or median (center), interquartile range (bounds of box), minima and maxima (whiskers) (d, e). p values were calculated by unpaired two-tailed Student’s t-test.
