Fig. 5: STAT3 degradation in DCs boosts immunity.
From: STAT5 and STAT3 balance shapes dendritic cell function and tumour immunity
a–e, Tumours in MC38-bearing NSG (a,b) and Rag1−/− mice (c–e) treated with SD-36 were monitored. n = 5 (a,b) and n = 6 (c,d); two-way ANOVA (a,c) and unpaired two-tailed t-test (b,d). Scale bar, 1 cm. NS, not significant. f, Tumour volume was monitored in MC38-bearing mice treated as indicated. Data are mean ± s.e.m., n = 6; **P = 0.0018, two-way ANOVA. g–l, FACS analysis of 4T1-infiltrating TNF+ (g,h), IFNγ+ (i,j) and GZMB+ (k,l) CD8+ T cells from mice treated with SD-36. Data are mean ± s.e.m., n = 6; *P = 0.0175 (h), *P = 0.0232 (j) and *P = 0.0404 (l), unpaired two-tailed t-test. m,n, Tumour volumes were monitored in Batf3−/− and wild-type mice bearing MC38 (m) or B16F10 (n) tumours and treated with SD-36. Data are mean ± s.e.m.; n = 5 (m), and n = 8 (n); *P = 0.0158 (m) and *P = 0.0126 (n), two-way ANOVA. o, FACS analysis of STAT3 expression in different cell types from LLC-bearing mice. Data are mean ± s.e.m., n = 4; ****P < 0.0001, one-way ANOVA. p–r, FACS analysis of pSTAT3 (p,q) and pSTAT5 (r) in MC38-infiltrating cDC1s from mice treated with SD-36. Data are mean ± s.e.m., n = 5; *P = 0.0335 and ***P = 0.0002, unpaired two-tailed t-test. s–u, FACS analysis of MHCI (s), MHCII (t) and CD80 (u) in B16F10-infiltrating cDC1s from mice treated with SD-36. Data are mean ± s.e.m., n = 5; **P = 0.0021 (s), **P = 0.0035 (t) and **P = 0.0023 (u), unpaired two-tailed t-test. v, Tumour volume in B16F10-bearing Stat3+/+ and Stat3−/− mice treated with SD-36. Data are mean ± s.e.m., n = 7; **P = 0.0067 and ***P = 0.0006, two-way ANOVA. w, B16F10-bearing Batf3−/− mice were transferred with Stat3+/+ and Stat3−/− cDC1s and treated with SD-36, and tumour volume was monitored. Data are mean ± s.e.m., n = 7; **P = 0.0094, two-way ANOVA. x, Stat5+/+ and Stat5−/− cDC1s were transferred into MC38-bearing Batf3−/− mice, treated with SD-36, and tumour volume was monitored. Data are mean ± s.e.m., n = 7; ****P < 0.0001, two-way ANOVA. y,z, Tumour volume was monitored in mice bearing MC38 (y) and B16F10 (z) tumours and treated as indicated. Data are mean ± s.e.m.; n = 6 (y) and n = 7 (z); **P = 0.0094 (z) and ***P = 0.0009 (y), two-way ANOVA.
