Extended Data Fig. 1: The phylogenetic tree of LICK family, MtLICK1/2 do not interact with MtNFP or MtDMI2 in Y2H.
From: A kinase mediator of rhizobial symbiosis and immunity in Medicago
a, Phylogenetic tree of LICK family members in Medicago truncatula, Glycine max, Lotus japonicus, Oryza sativa, Arabidopsis thaliana, Populus tremula, Vitis vinifera, Nicotiana benthamiana, Brassica napus, Sisymbrium irio, and Marchantia polymorpha. The phylogenetic tree was constructed using maximum likelihood method. ECD, extracellular domain. TM, transmembrane domain. KD, kinase domain. ECD, TM and KD were predicted by TMHMM-2.0. Extracellular domain and transmembrane domain were predicted by TMHMM-2.0. b, Yeast two-hybrid (Y2H) assay of MtNFP or MtDMI2 cytoplasmic domain, MtLICK1 and MtLICK2 with MtLICK1. Interaction of BK-MtLYK3CD and AD-MtLICK1 as a positive control. Empty vector (EV) was used as a negative control. c, Yeast two-hybrid (Y2H) assay of MtNFP or MtDMI2 cytoplasmic domain, MtLICK1 and MtLICK2 with MtLICK2. Interaction of BK-MtLYK3CD and AD-MtLICK2 as a positive control. Empty vector (EV) was used as a negative control. For b and c, the experiments were repeated three times with similar results.
