Extended Data Fig. 5: Validation of the MtPurF and HsPPAT enzymatic assays.
From: Targeting de novo purine biosynthesis for tuberculosis treatment
a, Schematic for enzymatic assays. b, SDS-PAGE gels showing MtPurF and HsPPAT protein purification. c, PurF reaction progress curves in HyPerBlu assay with 70 mM glutamine and 300 µM PRPP. n = 2 biological replicates. d, PPAT reaction progress curves in HyPerBlu assay with 200 µM glutamine and 30 µM PRPP. n = 2 biological replicates. e, IC50 values and fold selectivity from MtPurF and HsPPAT enzymatic assays. n = 2 biological replicates. Data shown are mean.
