Extended Data Fig. 10: Targeting WSTF in MASH.
From: WSTF nuclear autophagy regulates chronic but not acute inflammation
a and b, Related to Fig. 4a and b, additional images of WSTF staining of liver samples from control or MASH patients in cohort 1 and 2. c, Immunoblotting analysis of liver samples from mice fed on a chow diet or on the MCDE diet. d, Liver sections from chow diet or MCDE-treated mice were stained with WSTF and LAMP1 antibodies and analysed by a confocal microscopy. The colocalization of WSTF and LAMP1 is highlighted by arrows. WSTF signals in the MCDE group were deliberately overexposed to show the colocalization with LAMP1. e, Experimental design for establishing MASH in mice using MCDE model and scheme for administering peptides. f, Representative immunoblotting analysis of liver samples from mice fed on a chow diet or on the MCDE diet with NLS-CPP treatments. g, Quantifications of f (n = 8 animal samples). Mean and P values are shown; statistical analysis was conducted using unpaired two-tailed Student’s t-test. h, Representative images of IHC staining for WSTF, F4/80, α-SMA, and Sirius Red. i, Quantifications of IHC staining for h. n = 8 animal samples; data were from four randomly selected fields for each mouse. Results shown are the mean values; P values are shown as indicated, calculated by one-way ANOVA coupled with Dunnett’s post hoc test. j, RT-qPCR analyses of liver samples. The relative expression levels of pro-inflammatory genes and macrophage genes were measured. Results shown are the mean values; 8 mice in each group were used; P values are shown as indicated, calculated by one-way ANOVA coupled with Dunnett’s post hoc test. k, Liver sections were stained with an IL6 antibody then imaged using a confocal microscopy. Representative images are shown.
