Extended Data Fig. 5: HA-PCSPVY-aAtNRG1.1 confers resistance against multiple potyviruses.
From: Remodelling autoactive NLRs for broad-spectrum immunity in plants
a, Inoculated leaves of wild-type and T1 transgenic HA-PCSPVY-aAtNRG1.1 plants challenged with PVY-GFP. Photographs were taken under normal light and UV light at 7 dpi. HR lesions are indicated (arrows). Bar is 2 cm. b, Systemic tissues of PPV-inoculated WT and transgenic plants at 7 dpi. Bar = 5 cm. c, Inoculated leaves of T1 transgenic HA-PCSPVY-aAtNRG1.1 plants co-infected with TuMV-GFP, PPV-GFP, PepMoV and ChiVMV and wild-type plants infected with each of the four individual potyviruses. Two halves of one leaf of the transgenic plant were infected with TuMV-GFP and PPV-GFP, respectively. Two halves of another leaf of the same transgenic plant were infected with PepMoV and ChiVMV, respectively. One half leaf from different wild-type plants was infected with TuMV-GFP, PPV-GFP, PepMoV and ChiVMV, separately. Leaf photographs were taken at 7 dpi. HR lesions are indicated (arrows). Bar = 2 cm.
