Fig. 2: Tertiary interactions stabilize each ROOL_Efa monomer in the nanocage.

a, Molecular model of a ROOL_Efa monomer (Mono1) within the octameric structure. b–g, Close-up views of examples of tertiary interactions stabilizing the monomer are shown in the corresponding coloured boxes with the map rendered as a transparent surface (σ = 2.88): a Z-anchor (b), a 90° turn (c), the H12–H11 kissing loop (d), an H6–H8 interaction (e), an A-minor staple joining helices H4, H9, and H10 (f), a second A-minor staple involving these helices (g). h, Secondary structure annotation of ROOL_Efa within the octamer, with tertiary interactions labelled by coloured boxes that match panels b–g. Nucleotides in panels b–h are coloured according to helix colours in a.