Extended Data Fig. 3: Hunger delays and reduces parenting behaviors in virgin females and lactating mothers in the dark cycle.
From: A hypothalamic circuit that modulates feeding and parenting behaviours
a, schematic of the timing of fasting, refeeding, and conflict assay for fasted and refed groups. b, percent of animals that either performed pup retrieval or feeding as their first behavior (nvirgin = 7 mice, nlactating = 6 mice). c-f, probability density of pup retrieval and feeding events throughout the conflict assay (all trials). 1 total pellet consumed by 1 virgin female (refed) at time = 32.65 min is not shown to avoid skewing the visualization. g, transition probabilities between pup retrieval and feeding events in fasted virgin females and fasted lactating mothers. Only the feeding events prior to the completion of pup retrieval were used for the calculation. h, food intake. i, number of pups retrieved. j, latency to retrieve the first pup. k, nest score. l, preference index. m, food intake (# pellets) vs. % time in parenting chamber of virgin and lactating females in the fasted condition. Bar graphs show mean ± s.e.m. Box plots show median, upper/lower quartiles, and upper/lower extremes. Data analyzed using two-way RM-ANOVA with Šidák’s multiple comparisons test (h-l) or simple linear regression (m). Statistical details in Source Data.
