Extended Data Fig. 5: Altered immune responses and pathogen susceptibility are microbiome dependent.
From: Identification of medication–microbiome interactions that affect gut infection
a,b, Characterization of recipient mice after transplantation of gut microbiomes from donor C57BL/6NTac mice treated with digoxin or PBS for 2 days. Gene expression in ileal tissue of recipient mice (PBS n = 4, digoxin n = 3) as measured by qRT–PCR (a) and weight of recipient mice after infection with S. Tm ∆invA (n = 9 per group) (b). c,d, Characterization of recipient mice after transplantation of gut microbiomes from donor C57BL/6NTac mice treated with digoxin or PBS for 7 days. Weight of recipient mice (n = 5 per group) after infection with S. Tm ∆invA (c), and survival of recipient mice after infection with S. Tm ∆invA (d). e–g, Characterization of recipient mice (n = 5 per group) after transplantation of gut microbiomes from donor C57BL/6J mice treated with digoxin or PBS for 2 days. Weight of recipient mice after infection with S. Tm ∆invA (e), pathogen burden in faeces collected from recipient mice at 4 dpi (f) and survival of recipient mice after infection with S. Tm ∆invA (g). In a, fold change is measured relative to the mouse housekeeping gene, Gapdh. In a–c,e,f, a two-sided Mann–Whitney test is used to compare two groups. For survival analysis, the Gehan–Breslow–Wilcoxon test is used. Bar represents geometric mean in a, median in f and s.e.m. in b,c,e. ns, not significant.
