Extended Data Fig. 4: Effect of the duration of digoxin pretreatment on S. Tm infection.
From: Identification of medication–microbiome interactions that affect gut infection
a, Experimental design. CV C57BL/6NTac mice were treated with digoxin or PBS for one dose 2 h before infection (single-dose regimen), twice daily for 2 days followed by a 12-h washout period (standard regimen), or twice daily for 7 days followed by a 12-hour washout period (extended regimen). Mice were then infected with ~108 CFUs of S. Tm ΔinvA and infection monitored over time. b,c, Pathogen burden at 12 h after infection (n = 5 per group) (b) and mortality (c) after single-dose drug or control treatment. d–f, Pathogen burden at 12 h after infection (n = 5 per group) (d), mortality (e) and expression of proinflammatory marker genes in ileum tissue (n = 4 per group) (f) after the extended regimen drug or control treatment. g, Effects of digoxin or PBS treatment on the weight of CV C57BL/6NTac and C57BL/6J mice (n = 10 per group). Data are shown as mean with s.e.m. h, Cohoused C57BL/6J mice were separated from C57BL/6NTac mice prior to PBS or digoxin treatment and were then infected with a high dose (~109 CFUs) of S. Tm ΔinvA. Pathogen burden was enumerated at 3 dpi (n = 5 per group). In f, fold change is measured relative to the mouse housekeeping gene, Gapdh. In b,d,f,h, a two-sided Mann–Whitney test is used to compare two groups; bar represents median. For survival analysis, the Gehan–Breslow–Wilcoxon test is used. ns, not significant.
