Extended Data Fig. 3: De-N-glycosylation of cell surface RNAs makes apoptotic cells immunostimulatory.
From: RNA N-glycosylation enables immune evasion and homeostatic efferocytosis
(A) Efferocytosis of CellBright Red-labeled live or apoptotic HeLa cells treated with or without PNGase F, or PNGase F+RNase cocktail by CD11b+ peritoneal macrophages 2 hours post-feeding as assessed via flow cytometry. (B) IFN-β ELISA of RAW macrophage supernatants 24 hours post-stimulation with apoptotic HeLa cells treated with or without PNGase F or PNGase F+RNase cocktail at indicated macrophage:HeLa cell ratios. (C) IL-6 ELISA of peritoneal macrophage supernatants 24 hours post-stimulation with live or apoptotic HeLa cells treated with or without PNGase F or PNGase F+RNase cocktail at indicated macrophage:HeLa cell ratios. (D) TNF ELISA of peritoneal macrophage supernatants 24 hours post-stimulation with live or apoptotic HeLa cells treated with or without PNGase F or PNGase F+RNase cocktail at indicated macrophage:HeLa cell ratios. Data are represented as mean ± SEM (A–D). Pooled data from 3 independent experiments (A–D) are shown. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, not significant. Two-way ANOVA with Tukey’s test (A–D).
