Extended Data Fig. 1: Validation of CTCF CUT&RUN data.
From: Establishment of chromatin architecture interplays with embryo hypertranscription
a, Immunofluorescence of CTCF in mouse 1C, 2C, 4C, 8C embryos and blastocysts. Two independent experiments reported similar results. Scale bar, 20 μm. b, UCSC browser views showing CTCF binding with replicates for each stage. c, Scatter plots showing the CTCF enrichment levels (5-kb window) between biological replicates across the genome for each developmental stage. The Pearson correlation coefficients are shown. d, Pie chart showing (left) the percentages of common and variable CTCF sites across all stages in early embryos and (right) the percentage of variable peaks that either overlap with tissue CTCF peaks or contain CTCF motifs.
