Extended Data Fig. 8: Neuropathological assessment of npp tumours generated in wild-type and Sarm1-/- mice.
From: Axonal injury is a targetable driver of glioblastoma progression
a, Representative H&E wholemount images of terminal tumours generated in WT (i-v) and Sarm1-/- mice (vi-ix). Scale bar=2.5 mm (i, vi); =500 μm (iv, v and vii); = 250 μm (ii, iii); 100 μm (vii) 50 μm (ix). Black arrowheads indicate mitotic activity. Images are representative of 15 WT and 8 Sarm1-/- mice. b, Representative immunofluorescence images of npp terminal tumours generated in wild-type (WT) and Sarm1-/- mice and stained for Ki67 (grey). Tumour cells are identified by tdTomato fluorescence and nuclei are counterstained with DAPI (blue). Scale bar=200 μm. c, Quantification of percentage of Ki67+ tumour cells in tumours shown in b. n = 4 animals for both genotypes. Mean ± SEM. Two-sided unpaired t test. d, Representative immunofluorescence images of npp terminal tumours generated in WT and Sarm1-/- mice and stained for the endothelial marker CD31 (grey). Tumour cells are identified by tdTomato fluorescence (red) and nuclei are counterstained with DAPI (blue). Scale bar=200 μm. e-h, Quantification of indicated vascular phenotypes in tumours from d; n = 4 animals for each genotype. Mean ± SEM. Two-sided unpaired t test. in g, p = 0.0098; in h, p = 0.0464 i, Representative immunofluorescence images of npp terminal tumours generated in WT and Sarm1-/- mice and stained for the basal lamina marker laminin (red) and CD31 (green). Scale bar=200 μm. j, Quantification of the percentage of CD31+ vessels covered with laminin within tumours from i. n = 5 animals for each genotype. Mean ± SEM. Two-sided unpaired t test. k, Representative immunofluorescence images of npp terminal tumours generated in WT and Sarm1-/- mice and stained for of the pericyte marker PDGFRB (red) and CD31 (green). Scale bar=200 μm. l, Quantification of the percentage of CD31+ vessels covered with pericytes within tumours from k. n = 4 animals for each genotype. Mean ± SEM. Two-sided unpaired t test. m, Representative images of IgG extravasation (green) in npp terminal tumours generated in WT and Sarm1-/- mice. Tumour cells were identified by tdTomato fluorescence (red) and nuclei are counterstained with DAPI. Scale bar=200 μm. n, Quantification of IgG+ tumour area over total tumour area within tumours from m. WT n = 5, Sarm1-/- n = 4 mice. Mean ± SEM. Two-sided unpaired t test. o, Representative immunofluorescence images of npp terminal tumours generated in WT and Sarm1-/- mice and stained for Iba1 (green). Tumour cells are identified by tdTomato and nuclei are counterstained with DAPI (blue). Scale bar=200 μm. p, Quantification of the number of Iba1+ cells per mm2 tumour area in tumours shown in o. n = 4 animals for both genotypes. Mean ± SEM. Two-sided unpaired t test.
