Fig. 3: Designs occluding epitopes of common allergens.
From: One-shot design of functional protein binders with BindCraft
a, Left: design model of binder2 against dust mite allergen Der f7. Right: SPR binding affinity fit for binder2. b, Crystal structure (coloured) of the Der f7–binder2 complex overlaid with the design model (grey). c, Left: design model of binder10 against dust mite allergen Der f21. Right: SPR binding affinity fit for binder10. d, Crystal structure (coloured) of the Der f21–binder10 complex overlaid with the design model (grey). e, Left: design model of binder2 against birch allergen Bet v1. Right: SPR binding affinity fit for binder2. f, SEC–MALS analysis of Bet v1 allergen (blue, expected molecular weight (MW) 18.5 kDa) and Bet v1 mixed with binder2 (orange, expected molecular weight 29.3 kDa). g, Cryo-EM structure (PDB 7MXL) of Bet v1 bound to commercial anti-Bet v1 REGN antibody mix. h, Competition assay on immobilized REGN5713-Bet v1 complex binding of the REGN5714 antibody but not Bet v1 binder2, confirming binding at the designed site. i, Blocking ELISA showing the capacity of the REGN antibody mix (orange) or binder2 (blue) to prevent the binding of Bet v1 to IgE from the sera from three patients allergic to birch. Number suffix represents individual serum from a patient. Data points represent average of two technical replicates with the error bars depicting standard deviation.
