Extended Data Fig. 3: Targeting natural and de novo binding epitopes.
From: One-shot design of functional protein binders with BindCraft
a, Design model of binder4 in complex with de novo designed beta-barrel BBF-14. b, Single cycle kinetic analysis with SPR of binder4 binding to BBF-14. c, Comparison of crystal structure (coloured) of the BBF-14_binder4 complex overlaid with the design model (grey). d, Refined 2mFo − mFc electron density map of the BBF-14_binder4 complex rendered in gray and contoured at 1.0σ. The model complex refined against the map is shown as cartoon representation with BBF-14 coloured green and binder4 in salmon. e, Design model of binder4 binding to the challenging structural protein target SAS-6. f, SPR binding traces of binder4 to CrSAS-6 monomeric form. g, SPR binding traces of binder4 to CrSAS-6 dimeric form. h, Structural model of the oligomeric form of SAS-6 with binder4 (salmon) overlaid with the previously characterized monobody MBCRS6-122.
