Extended Data Fig. 9: Tumor-induced NRF2 signaling in BM limits T and NK cell activity In TME.
From: Myeloid progenitor dysregulation fuels immunosuppressive macrophages in tumours
a–b, Number of blood Ly6Ghi neutrophils in blood (a) and lung (b) of tumor-bearing Nfe2l2ΔMs4a3CreERT2 mice with early (n = 5) vs late deletion (n = 6) of NRF2. c, Representative gating strategy for major mouse lung lymphoid (T, B, NK cell) populations, with exemplar phenotypic markers depicted for CD8+ T cells and NK cells. d–e, Number of lung-infiltrating NK cells (d) and frequency of NK cells expressing CD69, NKG2D, and producing IFNγ (e) in KP tumor-bearing Nfe2l2ΔMs4a3 mice (n = 8) or negative littermates (n = 7) at day 21. f–g, Number of lung-infiltrating CD8+ T cells (f) and frequency of lung-infiltrating CD8+ T cells expressing PD-1, LAG3, CD69, or producing IFNγ, TNFα (g) in KP tumor-bearing Nfe2l2ΔMs4a3 mice (n = 8) or negative littermates (n = 7) at day 21. h, Representative histology and tumor burden for KP tumor-bearing Nfe2l2ΔMs4a3 mice and Nfe2l2fl/fl control that received anti-CSF1R depletion antibodies or isotype. n = 5 mice per group. i, Representative histology and tumor burden for KP tumor-bearing Nfe2l2ΔMs4a3 mice that received anti-NK1.1 antibodies or isotype. n = 7 mice per group. Unless noted, data shown as individual data points with bar denoting mean, representative of two independent experiments (a,b,d–g). Scale bar, 2 mm (h,i). Statistics computed by unpaired two-tailed Student’s t-test (a,b),(d–g),(i), one-way ANOVA with Sidak’s multiple comparison (h).
