Extended Data Fig. 13: CDP-choline treatment in cortical organoids.
From: ABCA7 variants impact phosphatidylcholine and mitochondria in neurons
a, Amyloid-β (Aβ40, Aβ42) levels quantified by ELISA from media of 4-week-old iNs (N wells=12 WT, 8 p.Tyr622*). Aβ40: two-sided t-test (equal variances); Aβ42: two-sided Mann–Whitney U test, following Shapiro–Levene tests. b, Representative images of cortical organoid slices by genotype; 5.5 months. c, Amyloid-β (Aβ40, Aβ42) levels quantified by ELISA from cortical organoid media (176-day-old), grouped by genotype and treatment (500 µM or 1 mM CDP-choline for 3 weeks). Samples correspond to organoids in Fig. 4k, analysed one week prior to assays presented there (N organoids=20 WT, 20 p.Tyr622*+H2O, 15 p.Tyr622*+500 µM CDP-choline, 14 p.Tyr622*+1 mM CDP-choline). Statistical comparisons (two-sided t-tests): Aβ40: WT vs p.Tyr622* (unequal variances), p.Tyr622*±500 µM (unequal variances), p.Tyr622*±1 mM (equal variances). Aβ42: all comparisons (equal variances). All tests followed Shapiro–Levene tests. a,c: boxplots=median, IQR (box), whiskers=1.5×IQR. a-c: experiments performed once.
