Extended Data Fig. 6: CRISPRa expression persists through 16 months post-systemic injection.
From: CRISPR activation for SCN2A-related neurodevelopmental disorders
Peak AP dV/dt (top) at 30-, 56-, 107-, and 210-days following tail-vein injection of Scn2a-rAAV-CRISPRa-PHP.eB in Scn2a+/−mice (purple). Circles represent individual neurons. Lines are linear regression. qPCR of mCherry (middle) or dCas9 (bottom) mRNA from neocortical samples collected from tail-vein or retro-orbital Scn2a-rAAV-CRISPRa-PHP.eB injected Scn2a+/+ (gray) or Scn2a+/− mice (purple) across time. Circles represent single mice. Lines are linear regression.
