Extended Data Fig. 5: Theoretical limit of detection in cfDNA.
From: Tracking clonal evolution during treatment in ovarian cancer using cell-free DNA
a) Detection probability as a function of cfDNA tumour fraction, number of mutations assayed and coverage using formula provided by Zviran et al.14 b) Theoretical limit of detection (probability of detection > 0.99) as a function of the number of mutations (x-axis) and Coverage (colors). Error rates of SNVs and SVs estimated in duplex and uncorrected sequencing from our data are shown on the left and right respectively. Star indicates relevant parameters for this study (100 mutations and 1000X coverage).
