Extended Data Fig. 3: Survey of inhibitory neuron initial classes in the developing mouse and investigation of the TAC3 initial class in Glire brains.
From: Conservation and alteration of mammalian striatal interneurons
a. UMAP coloured by developmental timepoint. b. Slingshot pseudotime calculation of developing mouse inhibitory neurons. c. Reconstruction of lineage trajectories with circles visualizing cell-type connections calculated using Slingshot. Owing to the enrichment of MGE precursors through use of a Nkx2-1-Cre;Ai14 reporter line, our mouse inhibitory neuron dataset contains more immature cell types compared to other samples and required more granular clustering for pseudotime and lineage analysis. d–o. Line graphs of gene expression within integrated MGE_CRABP1/TAC3 class across pseudotime for all developmental species. p. Image acquired at 20X of E17 Nkx2-1-Cre;Ai14 mouse brain, showing tdTomato reporter expression and Crabp1, Chrna3 RNAscope with inset indicating the highlighted region in the striatum (q). q. High-magnification (100X) image with arrowhead indicating the MGE_CRABP1/TH initial class. r. Image acquired at 20X of adult Nkx2-1-Cre;Ai14 mouse brain, showing tdTomato reporter expression and Crabp1, Chrna3 RNAscope with inset indicating highlighted region in the striatum (s). s. High-magnification (100X) image with arrowhead indicating MGE_CRABP1/TH initial class. t. Image acquired at 20X of adult Nkx2-1-Cre;Ai14 mouse brain, showing tdTomato reporter expression and Chrna3, Th RNAscope with inset indicating highlighted region in the striatum (u). u. High-magnification (100X) image with arrowhead indicating Th interneuron. v. High-magnification (100X) image of rabbit RNAscope with arrowhead indicating MGE_CRABP1/TAC3 class. w. Image acquired at 10X of adult rat RNAscope with markers Lhx6, Crabp1, and Tac3 and inset indicating highlighted region in the striatum (x). x. High-magnification (100X) image with arrowhead indicating no Tac3 expression in Lhx6/Crabp1-expressing cells. y. Image of naked mole rat striatum acquired at 40x with white arrowheads indicating no Tac3 expression in Lhx6/Crabp1-expressing cells and blue arrowhead indicating Tac3-expressing cell.
