Extended Data Fig. 8: Cryo-EM micrographs and 2D averages for CalC6_3 before and after DHR extensions.
From: Bottom-up design of Ca2+ channels from defined selectivity filter geometry
a-d, Design model (a), a representative cryo-EM micrograph of protein particles on a lacey carbon grid (b), and 2D class averages (c-d) from three different 2D classification settings, with an extraction box size of 300 pixels used in c and an extraction box size of 680 pixels (pixel size 0.4135 Å) used in d. e-i, Design model (e) and cryo-EM data (f-i) for CalC6 with DHR extensions. f and h, Representative cryo-EM micrographs of protein particles on a thin carbon grid (f) and a holey carbon grid (h), respectively. g and i, 2D class averages from the thin carbon grid (g) and from the holey carbon grid (h), respectively. Scale bars in the micrographs, 100 nm. Scale bars in c, 10 nm. Scale bar in d, 23 nm. Scale bars in i, 12 nm.
