Extended Data Fig. 9: Workflow for cryo-EM data processing of the CalC6_3 design with DHR extensions.
From: Bottom-up design of Ca2+ channels from defined selectivity filter geometry
The flowchart demonstrated particle picking, classification, reconstruction and refinement processes that enabled determination of both a hexamer and a heptamer structure. All data processing steps were carried out in CryoSPARC v4.4. Detailed processes can be found in Methods (2.11 and 2.12).
