Extended Data Fig. 2: Flux assay in HEK293T cells using Fura-2 AM.
From: Bottom-up design of Ca2+ channels from defined selectivity filter geometry
a-b (cyan), c-d (blue), and e-f (red) were data for CalC4, CalC6, CalC6_H designs, respectively. Ba2+ ions were added at the 900-s time point indicated by the vertical dashed lines. The gray traces represented measurements from mock-transduced cells as the negative control and were overlaid with the colored traces representing the cells transduced with the designs in each plot for comparison. b, d and f were close-up views of the plots highlighted by the red dashed frames in a, c and e, respectively, which were examples that were identified as the putative designs manifesting divalent ion permeability.
