Extended Data Fig. 3: Biophysical characterizations of more designed channels.
From: Bottom-up design of Ca2+ channels from defined selectivity filter geometry
In addition to the three designs shown in Fig. 2, two more CalC4 designs, CalC4_1 (a) and CalC4_4 (b), one more CalC6 design, CalC6_7 (c), and three more CalC6_H designs, CalC6_H1 (d), CalC6_H13 (e), and CalC6_H21 (f), were observed to elute as desired oligomeric states from SEC and assemble into homogeneous pore-containing particles on the ns-EM grids. The close-up views shown on the upper right corners of the ns-EM micrographs corresponded to the regions highlighted by the dashed yellow frames. Scale bars, 100 nm.
