Extended Data Fig. 7: Ca2+ deficiency promotes Capa secretion from the VaN.
From: Neuroendocrine control of calcium mobilization in the fruit fly
a, Schematic for rearing larvae on SHM or CFHM for Capa expression analysis. b, Relative Capa expression levels in larvae reared on SHM or CFHM for long durations (24 h). Capa expression levels do not change after 24 h on SHM or CFHM compared to 0 hAL3E. c, Schematic for rearing larvae on SHM or CFHM for Capa secretion analysis. d,e, Mature Capa peptide immunoreactivity in the VaN of larvae reared on SHM or CFHM for short durations (6 h). Representative images for each condition (d) and quantification of mature Capa peptide immunoreactivity (e) are shown. Mature Capa peptide immunoreactivity in the VaN decreased on CFHM but was restored upon SHM re-feeding, suggesting Ca2+ deficiency promotes Capa secretion from the VaN. w1118 was used in all experiments. Scale bars, 25 µm. Data shown as means ± SD. Sample sizes (n) and p values (p < 0.05) are shown in the graphs, with * indicating p < 0.0001 and ns denoting no significant difference (p > 0.05). Statistics: one-way ANOVA with Tukey’s multiple comparisons test (b,e).
