Extended Data Fig. 7: (Related to Fig. 4). Additional characterization of IAV-induced host cell Z-RNAs and ZBP1-bound RNAs.

a, Overlap between HSV-1- and IAV-induced Z-RNAs enriched in Z22 or FLAG pulldowns from FLAG-ZBP1 MEFs (left) or FLAG-ZBP1 HT-29 cells (right) infected with these viruses. b, Correlation between downstream transcription levels of individual mRNAs in FLAG-ZBP1 MEFs (left) and FLAG-ZBP1 HT-29 cells (right) infected with HSV-1 or IAV. Each mRNA is colour-coded based on the presence of virus-inducible Z-RNA downstream of the transcript. Normalized density plots of transcription levels for each mRNA class are shown on the marginal axes. The shaded area bounded by dashed lines represents mRNAs with similar downstream transcription levels (difference ≤ 10%) in both HSV-1- and IAV-infected cells. Only mRNAs meeting expression thresholds in both conditions are shown. The regression line for all mRNAs and its 95% confidence interval are shown in grey; r denotes the Pearson Correlation Coefficient. c, Proportion of proximal (within 10 kbp of TES) and distal ( > 10 kbp of TES) A → I intergenic editing sites identified in total RNA-seq from mock- or IAV-infected FLAG-ZBP1 MEFs (left) and FLAG-ZBP1 HT-29 cells (right). The total number of editing sites (both genic and intergenic) detected in each condition is indicated above the respective bars. Data are mean ± s.d. (n = 6, 6, 4, 8 biologically independent samples in order of conditions from left to right). d, Distribution of A → I editing sites in total RNA sampled at different time points from IAV- (A/Wyoming/03/03) infected human monocyte-derived macrophages. The total number of editing sites (both genic and intergenic) detected at each time-point is indicated on top. Sequencing data were obtained from the Sequence Read Archive (accession PRJNA382632).