Fig. 1: LN metastatic lines exhibit elevated FSP1 and reduced GCLC, GPX4 and ACSL4 expression.
From: Lymph node environment drives FSP1 targetability in metastasizing melanoma
a, Schematic of the generation of LN metastatic melanoma lines through serial in vivo selection across nine generations. The diagram was created using BioRender. b,c, The transcript levels of Gclc (b) and Fsp1 (c) across LN generations. TPM, transcripts per million. d, Immunoblot of ACSL3, ACSL4, GCLC, FSP1, xCT and GPX4 in B16-F0 (parental), early (LN1) and late (LN7–9) generations of LN metastatic lines. e–l, Individual (e,g,i,k) and grouped (f,h,j,l) quantifications of GCLC (e,f), FSP1 (g,h), GPX4 (i,j) and ACSL4 (k,l) protein expression levels across LN generations. Each replicate represents an independent experiment. For e–l, data are mean ± s.d. Statistical analysis was performed using one-way analysis of variance (ANOVA) followed by Dunnett’s test (e, g, i and k) and two-sided unpaired Student’s t-tests with Welch’s correction (f, h, j and l).
