Fig. 1: Gpx4 loss triggers ferroptosis in lung tumours.
a, Schematic of KP LUAD GEMMs intratracheally infected with pUSEC lentiviruses containing dual sgRNAs targeting Neo (control; n = 13) or Gpx4 (n = 13). Mice were dosed with LIP1 (sgNeo: n = 6, sgGpx4: n = 7) or vehicle (sgNeo: n = 7, sgGpx4: n = 6) every other day, starting from tumour initiation to experiment endpoint. b, Tumour burden of KP LUAD tumours with control (sgNeo) or Gpx4 (sgGpx4) knockout, treated with vehicle (Veh) or LIP1. c, Representative MRI of KP LUAD tumours with control (sgNeo) or Gpx4 (sgGpx4) knockout, treated with vehicle or LIP1. d, Representative haematoxylin and eosin (H&E) staining of KP LUAD tumours with control (sgNeo) or Gpx4 (sgGpx4) knockout, treated with vehicle or LIP1. Scale bars, 2,000 µm. e, Immunohistochemical detection of 4-HNE in KP LUAD tumours with control (sgNeo) or Gpx4 (sgGpx4) knockout, treated with vehicle or LIP1. f, Representative images of 4-HNE immunohistochemical staining from e. Scale bars, 20 µm. Data are mean ± s.e.m. One-way ANOVA with multiple comparisons (b,e). Drawing in a created in BioRender. Vaughan, A. (2025) https://BioRender.com/99qhixq.
