Extended Data Fig. 3: Neither Fsp1 nor Gpx4 have an impact on tumour initiation, proliferation, or apoptosis.
a, Quantification and representative images of tumour Fsp1 IHC in KP LUAD GEMMs with knockout of either control (Neo, n = 11) or Fsp1 (n = 14). Scale bars: 100 µm. b, Tumour number quantification in KP LUAD GEMMs with tumour-specific knockout of either control (Neo, n = 4), Fsp1 (n = 7), or Gpx4 (n = 6). c, Individual area of KP LUAD GEMM tumours with knockout of either control (Neo, n = 465), Fsp1 (n = 453), or Gpx4 (n = 384). d, Quantification of Ki67 IHC of KP LUAD GEMM tumours with knockout of either control (Neo, n = 355), Fsp1 (n = 268), or Gpx4 (n = 291). e, Quantification of cleaved caspase-3 IHC of KP LUAD GEMM tumours with knockout of either control (Neo, n = 46), Fsp1 (n = 22), or Gpx4 (n = 52). Data are represented as mean values, error bars represent SEM, significance determined via one-way ANOVA with multiple comparisons (panels b–e).
