Extended Data Fig. 5: CD8+TRM-like cells in healthy murine MFP.
From: Parity and lactation induce T-cell-mediated breast cancer protection
a, Relative expression of indicated expressed gene transcripts relative to GAPDH control isolated from MFP from virgin (n = 10) vs d28-inv (n = 9) timepoints from C57BL/6 mice. b, Representative OPAL multiplex images indicating the presence of DAPI+, CD3+, CD8+, CD103+ cells (white dots, depicts the co-expression of CD3, CD8 and CD103 surface markers on T cells) in the MFP of virgin, d10-FW and D28-inv C57BL/6 mice. Blue regions indicate local mammary draining lymph node and yellow represent mammary fat pad tissue boundary. c, Total number of CD3+CD8+CD103+ T cells in the 4th MFP of virgin (n = 7), d10-FW (n = 4) and d28-inv (n = 6) C57BL/6 mice measured by OPAL microscopy per mammary fat pad section. d, Representative OPAL images indicate the single-colour staining of DAPI, E-cadherin (ECAD), CD3, CD8 and CD103 on T cells in the MFP of virgin, d10-FW and D28-inv C57BL/6 mice. e, Mean distance between ECAD+ cells and the closest CD3+ CD8+ CD103+ T cells of virgin (n = 7), d10-FW (n = 4) and d28-inv (n = 6) C57BL/6 mice. Data in graphs represent mean ± s.e.m, results represent two combined independent experiments. Statistical significance determined by two sided Mann-Whitney test (a) or Kruskal-Wallis test (c, e). Exact p values are shown.
