Fig. 2: Complete lactation and involution significantly increased CD8+ T cells in healthy mouse mammary tissue.
From: Parity and lactation induce T-cell-mediated breast cancer protection
a, Experimental schematic of parity. b, Immune cell frequencies (%) among CD45+ cells in the MFP were analysed using flow cytometry analysis of virgin (n = 9) and d28-inv (n = 12) C57BL/6 healthy mice; CD8α+CD44high T cells are highlighted in green. c, The numbers of the indicated immune cell subpopulations per gram of MFP from virgin (n = 9) and d28-inv (n = 12) C57BL/6 mice. d, The numbers of the indicated immune cell subpopulations per gram of MFP from virgin (n = 8) and d28-inv (n = 8) BALB/c mice. e, The numbers of the indicated immune cell subpopulations per gram of MFP from virgin (n = 8) and d10-FW (n = 8) C57BL/6 mice. f, Uniform manifold approximation and projection (UMAP) analysis of CD8α+ T cells generated from flow cytometry data from the MFP of C57BL/6 virgin (n = 8) and d28-inv (n = 9) mice; three enriched are clusters indicated. Each dot represents an individual cell. g, Feature plots of the indicated markers on CD8α+ T cells projected on UMAP. Data are mean ± s.e.m. The results represent two combined biologically independent experiments. Statistical significance was determined using two-sided (c–e) and one-sided (f) Mann–Whitney U-tests. Exact P values are shown, or P < 0.0001 in cases in which P values are very small.
