Extended Data Fig. 8: iPEX uncovers early stage proteomic alterations during AD progression in 5xFAD mice.
From: iPEX enables micrometre-resolution deep spatial proteomics via tissue expansion
a, The brain anatomical structures of mouse brain coronal sections shown in Fig. 4a–d annotated from the Mouse Brain in Stereotaxic Coordinates 3rd Edition86. b, Ion intensity maps of selected ions that are dysregulated in the 5xFAD mouse brain. c, Information for Acaa1 precursor ions detected from the mouse cerebrum iPEX analysis. d, The MS2 spectra of the matched fragment ions for Acaa1 precursor ions identified in the mouse cerebrum. e, Normal MALDI validation for ion quantification in one 2-mo-old WT and one 5xFAD mouse brain regions including hippocampus (HPF), fibre tracts (FT), and cortex (CTX). f-g, IF image and quantification of Acaa1 (Acaa1a) protein expression and localization in brain sections of WT and 5xFAD mice at indicated ages. For quantification, each data point represents an ROI (see Methods section). P-values are calculated using unpaired, two-tailed Student’s t-test. n = 1399, 1717, 1686, 1447, 1270, 1138, 470, 548, 485, 472, 469, 743, 369, 426, 267, 346, 272, 412, 445, 560, 448, 344, 478, 634, 205, 193, 166, 246, 493, 253, 366, 442, 324, 297, 473, 456, 165, 156, 73, 119, 177, 163, 79, 27, 34, 47, 64, 69 ROIs for each bar. Scale bars indicated in images. Lines inside each violin represents quantile intensity values among pixels.
