Extended Data Fig. 10: Involvement of ACAA1 in fatty acid and membrane lipid metabolism in iPSCs-differentiated brain cell types.
From: iPEX enables micrometre-resolution deep spatial proteomics via tissue expansion
a, Induced differentiation strategies for generating cortical neurons, glutamatergic neurons and microglia from iPS cells. b, IF images of TUBB3 and VGLUT2 in cells differentiated from indicated WT and ACAA1 KO iPS cell SCPs. c, IF images of IBA1 and P2RY12 in cells differentiated from indicated WT and ACAA1 KO iPS cell SCPs. d, IF images of neural SOX2, TBR2 and CTIP2 in cells differentiated from indicated WT and ACAA1 KO iPS cell SCPs. e, Alterations in free fatty acid species between WT and ACAA1 KO iPSC-derived cortical neurons. f, Percentage distribution of lipid classes identified in the LC-MS/MS based lipidomics analysis in the indicated three lineages of iPSC-derived neural cells in negative ion mode (NEG). g-i, Indicated lipid species in the lipidomics analysis of phospholipid levels in iPSC-derived glutamatergic neuron (g), microglia (h) and cortical neuron (i) between WT and ACAA1 KO conditions in SCP#1 (left) and SCP#2 (right). Lipidomics data are presented as mean± s.d. (g,h,i). n = 3 biologically independent cell replicates for all conditions of glutamatergic neuron, ACAA1 WT microglia SCP#1 and all conditions of cortical neurons (g,h,i). n = 4 biologically independent cell replicates for ACAA1 KO microglia SCP#1 and all conditions of microglia SCP#2 (h). Scale bars indicated in images. The schematic in a was created using BioRender (https://www.biorender.com).
