Fig. 1: Inverse expression of three CSP pairs in the VA1d and VA1v glomeruli.
From: Repulsions instruct synaptic partner matching in an olfactory circuit
a, Schematics of adult Drosophila brain and the antennal lobe. Axons of VA1d-ORNs and VA1v-ORNs (green) match with dendrites of VA1d-PNs and VA1v-PNs (magenta), respectively. b, Schematic of conditional tagging of CSPs to reveal their endogenous protein expression pattern (top) before—and in specific cell types (bottom) after—FLP-mediated recombination. c,d, Confocal images showing neuropil staining (N-cadherin, blue) and MYC staining of tagged endogenous Ptp10D (red) (c) and Toll2 (cyan) (d) using ORN-specific FLP (top) or PN-specific FLP (bottom). VA1d and VA1v glomeruli are outlined in white and yellow, respectively, based on N-cadherin staining. Example images of other proteins are shown in Extended Data Fig. 2c. Asterisks indicate PN cell bodies. Scale bars, 10 µm. e–g, Quantification of the preference index of Toll2 and Ptp10D (e), Fili and Kek1 (f) and Hbs, Sns and Kirre (g) mRNA (black horizontal lines) and protein (coloured data points) expression levels in ORNs and PNs that innervate VA1d versus VA1v. A preference index above 0 means that the expression level in VA1d is higher than in VA1v. For all genotypes, n = 10 antennal lobes. Cyan or red lines indicate geometric mean. Whiskers extend to the most extreme data points within 1.5× the interquartile range. One-sample two-side t-test comparing to zero; P values are shown. h–j, Summary of relative expression of Toll2 and Ptp10D (h), Fili and Kek1 (i) and Hbs, Sns and Kirre (j) in the VA1d and VA1v PN–ORN pairs during development, based on mRNA and protein data in e–g (see Extended Data Fig. 2 for caveats). If the expression levels in VA1d- and VA1v-ORNs/PNs differ significantly, we only drew bars for the cell types with higher expression level as a simplification.
