Extended Data Fig. 7: Mistargeted VA1d-ORN axons in neighboring glomeruli are enriched for a presynaptic terminal protein.
From: Repulsions instruct synaptic partner matching in an olfactory circuit
Confocal images of adult antennal lobes showing neuropil staining by N-cadherin antibody (blue), VA1d-ORN axons (green, labeled by a membrane-targeted GFP) and a presynaptic active zone marker Bruchpilot-Short74 (Brp-Short, magenta). Knockdown of Ptp10D (a), overexpression of Kek1 (b), and overexpression of Kirre (c) in VA1d-ORNs caused their axons to mistarget to neighboring glomeruli (arrowheads). These mistargeted processes are enriched for Brp-Short, suggesting that mistargeted ORN axons might form synapses. Scale bars = 10 µm.
