Extended Data Fig. 11: Three CSP pairs are differentially expressed in other brain regions.
From: Repulsions instruct synaptic partner matching in an olfactory circuit
a, Confocal images showing neuropil staining by N-cadherin antibody (blue) and HA staining (green) of tagged endogenous cell-surface proteins in the Drosophila optic lobe at 42–48 h APF. The medulla (white dotted lines) and lobula (yellow dotted lines) are outlined based on N-cadherin staining. Arrowheads indicate the layers with differential expression patterns. For example, Kek1 is highly expressed in a medulla layer that is low for Fili expression. Scale bars = 50 µm. b, Confocal images showing HA staining (for Ptp10D, Kek1, and Hbs) and V5 staining (for Toll2, Fili, and Kirre) of tagged endogenous CSP pairs in the same brain (each row) at 42–48 h APF. The subesophageal zone, ellipsoid body, and protocerebrum regions are outlined based on neuropil staining. Scale bars = 50 µm. c, Pupal Drosophila brain schematic with brain regions in a and b highlighted in grey in the left hemisphere.
