Fig. 2: Loss of Ptp10D or Toll2 causes similar mismatching between non-partner PNs and ORNs.
From: Repulsions instruct synaptic partner matching in an olfactory circuit
a, Domain composition of Toll2 and Ptp10D. FN3, fibronectin type III; PTP, protein tyrosine phosphatase domain; TM, transmembrane domain; TIR, Toll/interleukin-1 receptor; SP, signal peptide. b–j, Left, experimental schematic. Right, confocal images of adult antennal lobes showing neuropil staining (N-cadherin, blue) and VA1d-ORN axons (green) (b–g) or VA1d-PN dendrites (magenta) (h–j). VA1v is outlined based on N-cadherin staining. Scale bars, 10 µm. b, Control VA1d-ORN axons innervate VA1d. c–g, Some VA1d-ORN axons mistarget to VA1v in Ptp10D hemizygous mutant flies (c), or in flies expressing Ptp10D RNAi in all (d) or individual (e) VA1d-ORNs, in Toll2 heterozygous mutant flies (f) or in flies expressing Toll2 RNAi in VA1v-PNs (g). h–i, Control VA1d-PN dendrites only innervate VA1d (h). Some VA1d-PN dendrites mistarget to VA1v when Ptp10D RNAi was expressed in VA1d-PNs (i) or when Toll2 RNAi was expressed in VA1v-ORNs (j). Asterisks indicate PN cell bodies. k,l, Penetrance of the mistargeting phenotypes in b–g (k) and h–j (l). n refers to the total number of antennal lobes examined. m, Schematic summary for the function of Ptp10D and Toll2. Inhibition arrow indicates repulsive signalling from sender to receiver.
