Fig. 4: Repulsive Fili–Kek1 interactions.
From: Repulsions instruct synaptic partner matching in an olfactory circuit
a, Domain composition of Fili and Kek1. Ig, immunoglobulin domain. b–d, Left, experimental schematic. Right, confocal images showing adult antennal lobe neuropil (N-cadherin, blue) and VA1v-ORN axons (green). VA1d is outlined based on N-cadherin staining. Scale bars, 10 µm. b, Control VA1v-ORN axons only innervate VA1v ventral to VA1d. c,d, Some VA1v-ORN axons mistarget to VA1d in kek1 mutant flies (c) or with kek1 RNAi expression in VA1v-ORNs (d). e, Penetrance of the mistargeting phenotypes in b–d. n refers to the total antennal lobes examined and is shown above the bars. f–k, Same as b–d, except VA1d-ORN axons (green) are visualized. DA1 and VA1v glomeruli are outlined based on N-cadherin staining. Scale bars, 10 µm. f, Control VA1d-ORN axons only innervate VA1d. g,h, Some VA1d-ORN axons mistarget to DA1 and VA1v following Kek1 overexpression in all (g) or individual (h) VA1d-ORNs. i, No VA1d-ORN axons mistarget to the DA1 or VA1v glomeruli in Fili mutant. j,k, Almost no VA1d-ORN axons mistarget to the DA1 and VA1v glomeruli when Kek1 overexpression in VA1d-ORNs was performed in Fili heterozygous (j) or homozygous (k) mutant. l, Penetrance of the mistargeting phenotypes in f–h. n refers to total antennal lobes examined and is shown above the bars. m, Quantification of the mistargeting ratio of VA1d-ORN axons in DA1 and VA1v glomeruli versus VA1d glomerulus. n refers to total antennal lobes examined. Boxes indicate geometric mean and 25th to 75th centiles and whiskers extend to the most extreme data points within 1.5× the interquartile range. Kruskal–Wallis test with Bonferroni’s multiple comparison. P values are shown. n, Schematic summary for the function of Kek1 and Fili.
